Sugar Processing Microbiology, Investigator: Day, D. F. LAB03556
Accession No: 0191192 Subfile: Cris
Proj No: Lab03556 Agency: Saes La.B
Proj Type: State Proj Status: Extended
Start: 01 Oct 2001 Term: 30 Sep 2007 Fy: 2006
Investigator: Day, D. F.
Performing Institution:
Audubon Sugar Institute
Louisiana State University
Baton Rouge, Louisiana 70893
Sugar Processing Microbiology
Classification
|
KA |
Subject |
Science |
Pct |
|
501 |
2020 |
1000 |
15 |
|
501 |
2020 |
1100 |
35 |
|
501 |
4099 |
1000 |
50 |
CLASSIFICATION HEADINGS: R501 . New and Improved Food Processing Technologies; S2020 . Sugar cane; F1000 . Biochemistry and biophysics; F1100 . Bacteriology; S4099 . Microorganisms, general/other
BASIC 25% APPLIED 40% DEVELOPMENTAL 35%
NON-TECHNICAL SUMMARY: Specific microbial groups cause processing problems in sugar production. Develop new compounds which can control or eliminate microbial sugar processing problems
OBJECTIVES: Development of solutions to problems in sugar manufacture that are of microbial origin.
APPROACH: Improve the use of biocides in sugar processing for microbial control. Develop new enzymes for control of deliterious microbial products with particular emphasis on polysaccharidases.
KEYWORDS: biocides; dextran; enzymes; sugars; food processing; food microbiology; product improvement; problem solving; bacteria; anti microbial agents; bacterial contamination; polysaccharidases; sugarcane; biochemistry
PROGRESS: 2006/01 TO 2006/12
A spin off of research into microbial modification of cellobiose, produced from bagasse, has resulted in the discovery of a new class of oligosaccharides. These compounds are showing a unique property of stopping growth of filamentous fungi. We wish to extend this project for one year, to investigate the potential of these compounds to inhibit growth of sugarcane fungal disease organisms, specifically those that cause sugarcane "smut" and "rust".
IMPACT: 2006/01 TO 2006/12
Prevention of fungal disease in sugarcane is a major focus of the sugarcane breeding program. Unchecked smut infections has the potential of decimating the Louisiana Sugar Industry. This has happened previously. There are few chemical fungicides available for agricultural use. A new "safer" chemical would be a welcome addition to the arsenal available to the producer. This new class of oligosaccharide holds potential to be such a compound.
PUBLICATIONS (not previously reported): 2006/01 TO 2006/12
- Kim, Duwoon and Donal F. Day. 2006 Synergistic Antimicrobial Action of Thymol and Sodium Bisulfate against Burkholderia cepacia and Xanthomonas maltophilia Isolated for the Space Shuttle Water System. Food Science and Biotechnology, 15, 321-323.
- Day, D. F. and P. Rein. 2006 Overview of the Audubon Sugar Institute Biorefinery Project. Annual Meeting ASSCT, La. Division, Baton Rouge, La. Feb 7-8.
- Kim, Misook, Chang-Ho Chung and Donal F. Day, 2006 Cellobiose Production using α-Glucosidase Inhibition. 28th Symposium on biotechnology for Fuels and Chemicals, Nashville, Tenn. Apr. 30 to May 3. Lee, Yong-Jae, Chang-Ho Chung and Donal F. Day, 2006 Oxidative Fractionation of Sugarcane Bagasse using a Combination of Hypochlorite and Peroxide. 28th Symposium on biotechnology for Fuels and Chemicals, Nashville, Tenn. Apr. 30 to May 3. Chang-Ho Chung, Donal F. Day and Giovanna A. DeQuerioz. 2006. Enzyme Hydrolysis and Phenols Recovery Post Alkaline and Organosolv Treatment of Sugarcane Bagasse. .28th Symposium on biotechnology for Fuels and Chemicals, Nashville, Tenn. Apr. 30 to May 3.
- Moon, Young-Hwan, Hyun-Ju Chung, Seung-Hee Nam, Doman Kim, Ghahyun J. Kim, Na-Young Woo, Ju-Bum Pyok and Donal F. Day. 2006. Characterization of Novel Epigallocatechin Gallate Glycosides using Glucansucrase from Leuconostoc mesenteroides B-1299CB. 28th Symposium on biotechnology for Fuels and Chemicals, Nashville, Tenn. Apr. 30 to May 3.
- Day, D.F., 2006 The use of enzymes in juice and syrup processing. ISSCT Processing Workshop, Baton Rouge, La. 29 May to 1 June.
- Day, D.F., 2006 Microbiological losses on mud handling. ISSCT Processing Workshop, Baton Rouge, La. 29 May to 1 June.
- Day, Donal F. 2006 Alternative Methods for Producing Ethanol from Sugarcane. 84th Annual meeting, Louisiana Farm Bureau, New Orleans, La. July 6-9.
- DeQueiroz, G. and D. F. Day. 2006. Phenolic acids released post-alkaline and/or organosolv treatment from sugarcane bagasse and field trash. 3rd Annual World congress on Industrial Biotechnology and Bioprocessing. Toronto, Canada, July 11-14.
- DeQueiroz, G. and Donal F. Day, 2007. Antimicrobial Activity and Effectiveness of a Combination of Sodium Hypochlorite and Hydrogen Peroxide in Killing an Removing Pseudomonas aeruginosa biofilms from Surfaces. J. Appl. Microbiol. IN Press
PROGRESS: 2005/01/01 TO 2005/12/31
Education focused on practical microbial control at the sugar mill, through direct inspection and control advice to the operating staff at each mill in Louisiana. Louisiana mills are now well aware of microbially generated problems and routinely apply control measures, improve sanitation and use biocides. Out-reach education will end with a short course this year on microbial problems in mud filters. Laboratory developments were a new cheaper method for dextran monitoring and biosafety through use of a new biocide developed in prior years. A Phage-display antibody based technology for monitoring dextran is now ready for commercial development, requiring an investor for commercialization. A trial of the biocide mentioned previously was conducted for controlling slime in a cooling tower at a commercial sugar mill. The biocide was very effective, but destroyed sugar, so it can only be used on contaminated surfaces. The technology has been licensed and is being marketed for other uses. Studies have been conducted on the effects of microorganisms on sugar loss in cane mud filters and control measures will be instituted in 2006.
IMPACT: 2005/01/01 TO 2005/12/31
In spite of crop variation, hurricanes and mill closures, Louisiana now produces almost half of the domestic cane sugar. Sugar mills have become more efficient than 5 or 10 years ago and currently natural gas usage is almost nil. Sugar mills now no longer wash cane, except in unusual circumstances. Microbially based problems, such as dextran are no longer an unsolvable problem but one that is handled routinely on the mill floor. More and more attention is being paid to minor losses in processing such that sugar recovery has increased an estimated 10 lbs per ton of cane processed. New products are relatively recent spin-offs from research at Audubon Sugar Institute. A commercially available test kit developed for dextran is in wide use in the sugar industry. In an early commercialization stage is a cold sterilant that has a wide range of applications, from military to infection control in hospitals, that came from research on developing a better biocide for sugar mills. The financial impact of these developments is in the range of 10-100 million dollars for the State.
PUBLICATIONS: 2005/01/01 TO 2005/12/31
- D. Kim and D. F. Day. 2005. A Dip-Stick Assay for Dextran Analysis. Proceedings of the XXV Congress, International Society of Sugar Cane Technologists. Atagua, Guatamala City, 2005 p 89-92.
- Madsen II, Lee R. and Donal F. Day. 2005. Mixed Dithiocarbamates for the Preservation of Sugar Cane Juice. International Sugar Journal 107:576-580.
- Donal F. Day. 2005. Biocide Compositions and related methods. US Patent 6,866,870 March 15.
- Donal F. Day and Chang-ho Chung, 2005 Chemical oxidation for Cellulose Separation, PTO serial # 60/660,801 filed 11 March 2005
- Chang-Ho Chung, Yong-Jae Lee and Donal F. Day. 2005. Chemical oxidation of bagasse for cellulose separation. 229th ACS National Meeting, San Diego, Calif. Mar. 13-17.
- C-H Chung, D. F. Day, M. Saska, P. Rein, T. Tiedje, F. Teymouri, D. McCalla and M. Stowers. 2005. Separation of cellulose from hemicellulose and lignin from sugarcane bagasse and cane leaf matter. 27th Symposium for Fuels and Chemicals. Denver, Co. May 1-4.
- D. Kim and D. F. Day. 2005. A Dip-Stick Assay for Dextran Analysis. XXV Congress International Society of Sugar Cane Technologists. Guatamala City, Guatamala. Jan 30- Feb 4.
- DeQueiroz, G. A., C. H. Chung, Y.J. Lee and D.F.Day. 2005. Process of turning sugarcane bagasse into cellulose and lignin-derived products. 35th Annual Joint Meeting, ASSCT. Panama City Beach, Fla. June 22-24.
- Kim, Misook, Zhimin Xu, Luis S. M. Bento and Donal F. Day. 2005. Antioxidant activities of methanol extracts from crude sugarcane syrup. IFT, New Orleans , July 15-19. New Orleans, La.
PROGRESS: 2005/01/01 TO 2005/12/31
Education focused on practical microbial control at the sugar mill, through direct inspection and control advice to the operating staff at each mill in Louisiana. Louisiana mills are now well aware of microbially generated problems and routinely apply control measures, improve sanitation and use biocides. Out-reach education will end with a short course this year on microbial problems in mud filters. Laboratory developments were a new cheaper method for dextran monitoring and biosafety through use of a new biocide developed in prior years. A Phage-display antibody based technology for monitoring dextran is now ready for commercial development, requiring an investor for commercialization. A trial of the biocide mentioned previously was conducted for controlling slime in a cooling tower at a commercial sugar mill. The biocide was very effective, but destroyed sugar, so it can only be used on contaminated surfaces. The technology has been licensed and is being marketed for other uses. Studies have been conducted on the effects of microorganisms on sugar loss in cane mud filters and control measures will be instituted in 2006.
IMPACT: 2005/01/01 TO 2005/12/31
In spite of crop variation, hurricanes and mill closures, Louisiana now produces almost half of the domestic cane sugar. Sugar mills have become more efficient than 5 or 10 years ago and currently natural gas usage is almost nil. Sugar mills now no longer wash cane, except in unusual circumstances. Microbially based problems, such as dextran are no longer an unsolvable problem but one that is handled routinely on the mill floor. More and more attention is being paid to minor losses in processing such that sugar recovery has increased an estimated 10 lbs per ton of cane processed. New products are relatively recent spin-offs from research at Audubon Sugar Institute. A commercially available test kit developed for dextran is in wide use in the sugar industry. In an early commercialization stage is a cold sterilant that has a wide range of applications, from military to infection control in hospitals, that came from research on developing a better biocide for sugar mills. The financial impact of these developments is in the range of 10-100 million dollars for the State.
PUBLICATIONS: 2005/01/01 TO 2005/12/31
- D. Kim and D. F. Day. 2005. A Dip-Stick Assay for Dextran Analysis. Proceedings of the XXV Congress, International Society of Sugar Cane Technologists. Atagua, Guatamala City, 2005 p 89-92.
- Madsen II, Lee R. and Donal F. Day. 2005. Mixed Dithiocarbamates for the Preservation of Sugar Cane Juice. International Sugar Journal 107:576-580.
- Donal F. Day. 2005. Biocide Compositions and related methods. US Patent 6,866,870 March 15.
- Donal F. Day and Chang-ho Chung, 2005 Chemical oxidation for Cellulose Separation, PTO serial # 60/660,801 filed 11 March 2005
- Chang-Ho Chung, Yong-Jae Lee and Donal F. Day. 2005. Chemical oxidation of bagasse for cellulose separation. 229th ACS National Meeting, San Diego, Calif. Mar. 13-17.
- C-H Chung, D. F. Day, M. Saska, P. Rein, T. Tiedje, F. Teymouri, D. McCalla and M. Stowers. 2005. Separation of cellulose from hemicellulose and lignin from sugarcane bagasse and cane leaf matter. 27th Symposium for Fuels and Chemicals. Denver, Co. May 1-4.
- D. Kim and D. F. Day. 2005. A Dip-Stick Assay for Dextran Analysis. XXV Congress International Society of Sugar Cane Technologists. Guatamala City, Guatamala. Jan 30- Feb 4.
- DeQueiroz, G. A., C. H. Chung, Y.J. Lee and D.F.Day. 2005. Process of turning sugarcane bagasse into cellulose and lignin-derived products. 35th Annual Joint Meeting, ASSCT. Panama City Beach, Fla. June 22-24.
- Kim, Misook, Zhimin Xu, Luis S. M. Bento and Donal F. Day. 2005. Antioxidant activities of methanol extracts from crude sugarcane syrup. IFT, New Orleans , July 15-19. New Orleans, La.
PROGRESS: 2004/01/01 TO 2004/12/31
Research focused on practical microbial control at the sugar mill, through direct inspection and control advice to the operating staff at each mill in Louisiana. Laboratory developments were on new cheaper methods for dextran monitoring and biosafety through use of a new biocide developed in prior years. A Phage-display antibody based technology for monitoring dextran is now ready for commercial development, requiring an investor for commercialization. A trial of the biocide mentioned previously was conducted for controlling slime in a cooling tower at a commercial sugar mill. It will be repeated in 2005. Studies have been initated on the effects of microorganisms on sugar loss in cane mud filters.
IMPACT: 2004/01/01 TO 2004/12/31
Sugar is a major component of Louisiana's agricultural sector. The direct value of this crop, not including value added calculations is in excess of $640,000,000. Microbial losses during sugar production are small but not trivial, and range from sugar loss due to undesirable polysaccharides to equipment loss due to corrosion from microbially generated acids. Control of these losses can significantly improve the operating costs of sugar mills. Dextran is a continuing economic problem in sugar production. The primary source is from stale cane. The ability to rapidly detect stale cane as it reaches the sugar mill, prior to processing, would be of great value. The volume of analyses required needs a lower cost alternative analytical method than is currently available.
PUBLICATIONS: 2004/01/01 TO 2004/12/31
- Duwoon Kim and Donal Forest Day. 2004. Determination of Dextran in Raw Sugar Process Streams. Food Science and Biotechnology. 13, #1, 1-5.
- DeQueiroz, G. A. and D.F. Day. 2004. Effectiveness of a bi-component Food Grade Sanitizer in the Removal of Pseudomonas aeruginosa Biofilms from Surface Materials.104th Annual Meeting, American Society for Microbiology. New Orleans, La. May 23-27.
- Day, D. F. and D. W. Kim. 2004. Dextran Dip-Stick: A Model for Assay Development. 34th Annual Joint Meeting American Society of Sugar Cane Technologists. St. Petersburg, Fla., June 16-18.
- Madsen II, L.R. and D. F. Day. 2004. A Method for Correcction of Pol Error in Sugar and Juice. 34th Annual Joint Meeting American Society of Sugar Cane Technologists. St. Petersburg, Fla., June 16-18.
PROGRESS: 2003/01/01 TO 2003/12/31
Research was conducted on the effect of organic acids on sugar boiling, microbial contamination at filter stations, and education of the sugar mills on proper sue of the enzyme dextranase in thier processing. Both the organic acid and filter station research will be continued into next season. This season some spots of microbial contaimination were found in filter stations. Data collection was limited on the organic acid effects such that further collection is warrented. All mills are now using dextranse when and where appropriate. There was little dextran reproted this season so it is not possible to judge the effectiveness of each mills operation.
IMPACT: 2003/01/01 TO 2003/12/31
Dextran has been a long term problem, it is part of an overall problem of poor sugar cane quality and its effects on sugar production. Control of sugar cane quality and the microbial effects brought about by poor cane will have a multimillion dollar effect on this industry.
PUBLICATIONS: 2003/01/01 TO 2003/12/31
- Kampen, W. H. and D. F. Day. 2003 Organic Acids (+). Annual Meeting ASSCT, Louisiana Division, Baton Rouge, La. Feb 4-5.
- Kim, D.W. and D. F. Day. 2003 Selection of a dextran binding antibody using a phage display library. 103rd Annual Meeting, American Society for Microbiology, Washington, D. C. , May 19-23 .
PROGRESS: 2002/01/01 TO 2002/12/31
This research concentrates on defining problems of microbial origin that affect the production of raw cane sugar and developing practical solutions to those problems. Three areas of microbial control were addressed. First, the proper use of commercial biocides was demonstrated; second, many of the problems in the processing of sugar cane juice were found to be associated with microbial products produced from re-cycled cane wash water, including significant corrosion; and third, the MCA-dextran test kit was demonstrated to be an appropriate method for factory control with regard to use of dextranase.
IMPACT: 2002/01/01 TO 2002/12/31
Sugar is a major component of Louisiana's agricultural sector. The direct value of this crop, not including value added calculations is in excess of $640,000,000. Microbial losses during sugar production are small but not trivial, and range from sugar loss due to undesirable polysaccharides to equipment loss due to corrosion from microbially generated acids. Control of these losses can significantly improve the operating costs of sugar mills.
PUBLICATIONS: 2002/01/01 TO 2002/12/31
- Day, D.F., 2002. Corrosion in the Sugar Factory. Annual Meeting, ASSCT, Louisiana Division, Feb 26-27, 2002, Baton Rouge, La.
- Day, D.F. and Cuddihy J., 2002. Biocide Usage at Louisiana Factories. Annual Meeting, ASSCT, Louisiana Division, Feb 26-27, 2002, Baton Rouge, La.
- Saska, M., Godshall M.A. and Day D.F., 2002. Dextran analysis with polarimetric, immunological, Roberts and haze methods. Annual Meeting SPRI, New Orleans, La. , March 11-13.
- Day, D.F., Cuddihy J. and Rauh J., 2002. Versatility of the Antibody Dextran Test Method. 32nd Annual Joint Meeting, American Society of Sugar Cane Technologists, Amelia Island Plantation, Fla. June 26-28.
- Day, D.F. and Kampen W.H., 2002. Organic Acids in the Sugar Factory Environment. 32nd Annual Joint Meeting, American Society of Sugar Cane Technologists, Amelia Island Plantation, Fla. June 26-28.
PROJECT CONTACT:
Name: Day, D. F.
Phone: 225-578-3348
Fax: 225-578-2708
Email: dday@agctr.lsu.edu
SUPPLEMENTARY DATA: Institution Type: SAES Region: 2 Process Date: 2001/11/28 Progress Update: 2007/04/02
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